DETERMINATION OF PHOSPHORYLASE ACTIVITY IN THE PRESENCE OF β-AMYLASE

Abstract

Detailed proccclures are given for the quantitative determination of phosphorylase activity in the presence of potent β-amylase. This method is based on the finding that β-amylase is more sensitive to mercuric chloride than is phosphorylase. By correcting the observed phosphorylase activity in the presence of a suitable concentration of mercury for the recovery of added phosphorylase in the same digest, true phosphorylase activity is obtained. It was found by this method that crude extracts from sweet potatoes are very potent in phosphorylase activities.
The author wishes to express his sincere thanks to Prof. S. Funahashifor his interest in this work and to Mr. M. Onoda for the generous gift of various varieties of sweet potatoes.