Abstract
The incorporation of P32 into the phospholipid and the residual fractions of avian tuberble bacilli was studied under various conditions. By the addition of inorganic phosphate and under anaerobic condition, the incorporation rate of P32 was reduced. The incorporation rate increased after treating the bacilli in veronal buffer for 4 hours at 37°.
The effect of various inhibitors, cofactors, antibiotics metal ions upon P32-incorporation was also investigated and the mechanisms of the process were discussed.
This work was carried out under the direction of Prof. K. Hoshishima to whom the author wishes to express his thanks for the constant guidance in the course of this work.
I wish to express my sincere gratitude to Dr. K. Matsui, National Sanatorium Toneyama Hospital, for supplying us the avian tubercle bacillus, strain Takeo. I am also indebted to Dr. T. Shirotori, Toa Alimentary Chem. Ind. Co., Ltd., for his kindness to furnish us a sample of FAD.
Thanks are due to Prof. M. Umezu and Prof. T. Uemura, Faculty of Agriculture of Tohoku University, Sendai, and to Prof R. M. C. Dawson, Biochemistry Department, A. R. C. Institute of Animal Physiology, Babraham, Cambridge, England, for their invaluable advice and criticism.
