Abstract
1. Amino acids and lower peptides could be TNP-lated both with CITNB and with TNBS.
2. TNBS coupled with primary amino group but not with water nor other groups commonly encountered in protein, in water solution at pH 8. and at room temperature.
3. The preparation and some properties such as m. p., Rf, molar extinc-tion coefficient, photo-and acid-stabilities, of TNP-amino acids and -peptides. (α-mono-TNP-Gly, -L-Ala, -L-Val, -L-Leu, -L-Tyr, -L-Arg, -L-Asp, -L-Glu, L-Met, -L-His, ε-mono-and α, ε-Bis-TNP-Lys; α-mono-TNP-Gly-Gly, -Gly-DL-Val, Gly-DL-Ser, -DL-Ala-DL-AspNH2 and -L-Leu-L-Tyr) are reported, together with the ultraviolet and infrared absorption spectra of some typical TNP derivatives.
4. These results were briefly discussed with connection of possible application of TNBS in protein chemistry.
We are greatly indebted to Mrs. S. Sasakawa, Mr. H. Ozawa and Mr. T. Take, for their unpublished data. We wish to express our gratitude to Mr. K. Nitta, Mr. M. Ohashi, Mr. H. Shino and Mr. T. Shinoda for their technical assistance in the preparation of various TNP compounds.
These studies were supported in part by a grant from the Scientific Research Fund of the Ministry of Education. The authors should express their sincere thanks to them.
