STUDIES ON ENZYMIC NITRITE REDUCTION

III. EFFECTS OF METAL IONS ON SOLUBLE AND PARTICULATE COMPONENTS OF NITRITE REDUCTASE

Abstract

1. The particulate component (PF) of nitrite reductase of a halotolerant micrococcus, strain 203, was solubilized by the treatment with snake venom and purified by ammonium sulfate fractionation.
2. Using partially purified and thoroughly dialyzed preparations, it was found that the activity of soluble component (SF) is activated by Cu⁺ and Cu⁺⁺ and that of particulate component (PF) is enhanced by Fe⁺ and Fe⁺⁺⁺.
3. Intracellular distribution of CO-binding pigment(s) in this organism was studied.
The author wishes to thank Prof. F. Egami for his kind encouragement and guidance. Thanks are also due to Prof. R. Sato, Institute for Protein Research, Osaka University, for his kind criticism on the manuscript and to Mr. H. Maeno, Gunma University Medical School, for the supply of snake venom.