Abstract
1. From the pupae of the silkworm, Bombyx mori, an enzyme which specifically hydrolyzes trehalose, has been purified.
2. Chromatographic procedure has revealed the existence of two forms of trehalase, but their properties did not differ as fas as trehalose hydrolysis is concerned.
3. In a larval stage, trehalase activity is found in intestine, body-wall muscle, and fat body, but not in body fluid and silk gland.
The author wishes to express his gratitude to Prof. Y. Akita and Assist. Prof. M. Akino for their guidance and criticism throughout this work. Thanks are also due to Prof. K. Dan for reading the manuscript.