Abstract
Ester and amide of carbobenzoxy-L-glutamic acid-γ-hydrazide, a lysine analogous compounds, have been synthesized and tested their nature as substrate for trypsin. These compounds can be partly hydrolysed by the enzyme action. Optimum pH, Km and proteolytic coefficient k3 were also determined for the hydrolysis. Peptide and polymer containing L-glutamic acid-γ-hydrazide residues were also slowly hydrolysed by the action of the enzyme, but those containing D-isomer were not. The inhibition of the tryptic activity npon usual substrate by glutamic acid-γ-hydrazide compounds were studied.
The author wishes to express his hearty thanks to Prof. S. Akabori and Dr. A. Tsugita of our University, for their interest and encouragement throughout the course of this work.
