Enzymatic Studies on Pyridoxine Metabolism

VII. The Influence of Fat and Fatty Acid Administration upon Pyridoxine Metabolism

Abstract

Weekly determination on rats fed on a high fat diet for 4 weeks revealed a gradual decrease in the pyridoxal phosphate content and pyridoxine phosphate oxidase activity. This was prevented by the simultaneous ad-ministration of FMN. Similarly, oral admi-nistration of butyrate caused a decrease in the pyridoxal phosphate content and pyridox-ine phosphate oxidase activity. Caproate, stearic acid and palmitic acid had similar effects. The decrease was prevented by the simultaneous injection of riboflavin. In these cases the depression of pyridoxal phosphate content was probably due to a decrease in the pyridoxine phosphate oxidase activity.