Abstract
The oxygen uptake by nuclear, mitochon-drial, and supernatant fractions of rat liver in D-amino acid oxidase reaction mixture was measured. Then the apo-enzyme of n-amino acid oxidase was purified from each fraction, and the activity was tested.
The ratio of the activities of D-amino acid oxidase purified from the nuclear, mitochon-drial, and supernatant fractions was nearly the same as that of the activities of these fractions themselves.
The ratio of the enzyme activity in these subcellular fractions was corrected by the contamination of the mitochondria, calculated by the ratio of succinic dehydrogenase activity.
The results showed that the larger amount of D-amino acid oxidase exists in the mitochon-dria, and the small amount in the supernatant.
