Abstract
The microbiological determination of L-isoleucine and the total amount of isoleucine isomers by empoying the adapted strain of Lact. arabinosus 17-5 is described.
This assay method is found to be very convenient and satisfactory for the determination of total isoleucine isomers in amino acids mixtures and this method also can be applied to the assay of the proportion contaminated with allo-form in synthetic isoleucine preparations.
The authors wish to thank Prof. S. Akabori and Assist. Prof. Y. Izumi, Osaka University, for their interest and helpful advice throughout this work. The authors are also greatly indebted to Mr. T. Hira-bayashi, Representative Director of the Tanabe Seiyaku Co. Ltd., Dr. M. Fujisawa, Research Executive and Dr. N. Sugimoto. Director of Osaka Research Laboratory, for continued encouragement and assistance.
