Inhibition in Vitro of Growth of Rat Ascites Tumor Cells by Bovine Liver Extract
Purification and Property of the Actice Principle
1962 Volume 51 Issue 5 Pages 322-334
Details
1962 Volume 51 Issue 5 Pages 322-334
An active principle which inhibited in vitro the growth of rat ascites tumor cells was extracted from adult cattle liver tissue with physiological saline or salt-free water.
The principle was concentrated from the extract by a stepwise salting-out treatment with ammonium sulfate at 1/2 saturation, by the removal of inactive proteins denatured by heating at 56°C for 30 minutes and by a single application of Sevag's procedure.
Upon subjecting the concentrated fraction to DEAE-cellulose column chromatography, the active protein fraction was separated from the inactive fractions.
The electrophoretic mobility of the active fraction was studied.
Some discussions as to the mode of the activity in cell-growth inhibition of this compound were made.
The authors are indepted to Dr. H. Katsuta, Assist. Prof. of the Institute for Infectious Diseases, the University of Tokyo, for his advices in cell cultivation technics, and to Dr. H. Inaamura and Mrs. A. Moriwaki of Cancer Chemotherapy Section, Sasaki Institute for their co-operation in experiments.
A part of the expense for this work was defrayed by Grant-in-Aid for Scientific Research provided by the Ministry of Education.
