Abstract
1. The conditions of the fractionation of proteins and nucleic acids of Pseudomonas-P on DEAE-cellulose column chromatography and sedimentation analysis of the fractions were established.
2. Amino acids incorporation experiments revealed that the uniform protein synthesis occurred in exponentially growing cells, and a heterogeneous synthesis was observed in subcellular fraction (MgP) from Pseudomonas-P.
3. Comparison was made on DEAE-cel-lulose column chromatogram and patterns of sedimentation analysis of the fractions from exponentially growing cells and from MgP.
4. MgP synthesized two types of proteins which were eluted with lower concentrations of NaCI and 0.5N KOH, respectively. The bulk of the free proteins which usually were present in intact cells were absent in the products by MgP.
5. The nascent proteins were loosely attached to ribosomes and released upon lowering the concentration of magnesium ion in solution or upon treating them with DEAE-cellulose.
The authors wish to express their many thanks to Prof. S. Akabori, the President of the Osaka University, for his kind encouragement in this work. Thanks are also due to Mr. S. Mizuno, in this institute and Dr. H. Mitsui, Kanazawa University, for their valuable discussions and advices through this work. The authors are indebted to Miss T. Hattori of this institute for the preparation of C14-amino acids.
