Abstract
1. Streptolysin S' was purified from supernatants of resting cell suspensions of Streptococcus pyogenes in a medium with oligoribonucleotides from ribonuclease I core of yeast ribonucleic acid. By successive applica tion of zone electrophoresis and DEAE-cellulose column chromatography, purified toxin with 100, 000 HU per optical density at 260mμ was obtained with good reproducibility.
2. The purified preparation thus obtained was found to contain nucleotides and amino acids.
3. From these results, it was concluded that streptolysin S' molecule may be a complex containing both oligoribonucleotides and a peptide.
The present authors wish to express their sincere thanks to Dr. H. Ishikura for his useful discussions and Dr. Y. Homma of the Institute for Infectious Diseases, University of Tokyo for his kindness to facilitate this work.
