Intracellular Transfer of Nucleic Acids

II. Effect of Hormones on Ribonucleic Acid Metabolism in Rat Liver Cells

Abstract

1. The in vivo uptake of P³²_i into nuclear RNA of male rat liver was compared with that of the same fraction of female rat liver. The female rat liver incorporates more P³²_i, into nuclear RNA than the male rat liver.
2. Enhancing effects of estradiol and inhibitory effects of testosterone on the uptake of P³²_i into nuclear RNA were found in castrated rat liver. Tentative point of the hormonal action was postulated.
3. Hypophysectomy did not alter the incorporation rate in female rats, however, administration of growth hormone apparently increased the uptake of P³²_i. β-Androstanolone did not act as androgen, it gave rather an estrogen-like effect on male rats.
This study was supported by research grants form the Ministry of Education (Japan) and the National Institures of Health, U.S.A. (CY-5869 CY).