Abstract
The molecular weight of M-PhAB-TAA was estimated by sedimentation, diffusion and partial specific volume measurements and also by determination of the amount of PhAB-group bound with the modified enzyme. The value of 36, 000 was estimated by the former method and 35, 000 by the latter.
Alteration of catalytic properties due to the partial degradation of intact PhAB-TAA was examined and discussed in view of changes of kinetic parameters and thermody-namic quantities.
The author wishes to express her thanks to Prof. S. Akabori, Prof. K. Narita and Dr. T. Ikenaka for their kind guidances and valuable discussions through this investigation and also to Sankyo Co., Ltd. for their kind supply of “Takadiastase Sankyo.”
