Studies on the Ribonuclease of Streptomyces aureus

I. Purification and General Properties

Abstract

The ribonuclease capable of decomposing RNA was purified up to an electrophoretically and Resin-chromatographically homogeneous state from the medium filtrate of Streptomyces aureus. The enzyme was remarkably activated by Mn_??_and Mg_??_but inhibited by EDTA. Optimum pH was ranged from 6.5 to 9.0. The enzyme had a different specificity from those of pancreatic RNase, ribonuclease T₁ and Streptomyces ribonuclease.
The author wishes to express his thanks to Dr. E. Ohmura for fruitful discussion and Mr. H. Tanaka and Mr. Y. Kihara for technical assistance.