Quantitative Determination of Anomeric Forms of Sugar Produced by Amylases

III. Taka-Amylase A-Phenyl-α-maltoside System

Abstract

Hydrolytic reaction of phenyl α-maltoside catalyzed by crystalline Taka-amylase A was carried out at pH 5.1 (0.05M acetate buffer) and at 35°C. By means of simultaneous measurement of reducing power, amount of phenol and optical rotation, the time courses of α-and β-maltose, phenol and phenyl α-maltoside of the reaction system have been obtained.
It has been demonstrated that α-maltose is released exclusively from this substrate.