Concentrations of Intracellular Glycolytic Intermediates and Nucleotides Dihydroxyacetone-fermenting Cells of Brevibacterium fuscum

Abstract

1. Br. fuscum was cultured for 22 hours at 30°C with shaking in a 5% glucose medium, and the cytoplasmic volume of the cell was measured by electron microscope.
2. Glycolytic intermediates and nucleo-tides were extracted with TCA from the cell and their levels in the cytoplasmic fraction were evaluated after ion exchange chromato-graphic procedures.
3. The experimental results showed that, generally, the amounts of free sugars such as glucose, DHA, ketohexose, etc. rose above those of the other phosphate esters.
4. FDP was found to be accumulated at the highest level of all the glycolytic sugar phosphates, and followed by 2-PGA and G 6 P.
5. It was also observed that cytoplasmic solution abounded in phosphorus compounds such as ADP, Pi, ATP, UDP and its com-plexes, NAD, GTP, etc.
The author wishes to express his sincerest thanks to Prof. K. Arima and Prof. Y. Ikeda of the Univer-sity of Tokyo for their kind guidances throughout this work. Great indebtedness is also acknowledged to Dr. S. Mizushima for his valuable suggestions.
The author also wishes to thank Dr. M. Mogi and Dr. N. Iguchi for their kind advices and en-couragements.