Abstract
Ascorbate oxidase [EC 1. 10. 3. 3] was purified from cucumber, Cucumis sativus, and its molecular weight and other physicochemical properties were investigated. This enzyme contains 8 atoms of copper per molecular weight of 132, 000 and has a specific activity of 3, 500 Dawson's units/mg. Results on spectrophotometric and ESR measurements, as well as those on kinetic analysis and azide inhibition of the enzyme, are also presented.
