Abstract
The possibility of applying isotope dilution analysis in determination of individual sphingoglycolipids was examined. Tritium labelled sphingoglycolipids were prepared by catalytic reduction with tritium. The labelled compounds were clearly separated from unlabelled compounds on silicic acid column and silica gel thin layer chromatographies. This was found to be due to the disappearance of double bonds in the sphingosine bases and fatty acids during the reduction procedure. There fore, sphingoglycolipids should be determined by isotope dilution analysis using samples in which the double bonds have first been saturated by catalytic reduction.
