Abstract
1. Accumulation of arsenate-76 by rat liver mitochondria was measured to study phenomena which are not explained by the arsenolysis of a high energy intermediate.
2. Stimulation of ATPase [EC 3. 6. 1. 3] or respiration of mitochondria by arsenate was accompanied by its accumulation. This reaction required energy and a divalent cation.
3. Respiration-dependent arsenate accumulation required adenine nucleotide and was inhibited by atractyloside but was stimulated by oligomycin. These were the marked differences from phosphate accumulation.
4. The relationship between arsenate accumulation and phosphorylating mechanism is discussed, since effects of arsenate have been considered to support the presence of a high energy phosphorylated intermediate.
