Studies on Ribonucleases from Physarum polycephalum Purifiation and Characterization of Substrate Specificity
1969 Volume 65 Issue 5 Pages 693-700
Details
1969 Volume 65 Issue 5 Pages 693-700
1. Three RNases were isolated from Physarum polycephalum and designated RNase Pp-1, RNase Pp-2 and RNase Pp-3.
2. They are thermostable.
3. The approximate molecular weights estimated by get filtration of RNases Pp-1 and Pp-2 are 40, 000 and that of RNase Pp-3 is 10, 000.
4. The optimum pH values of RNases Pp-1, Pp-2 and Pp-3 are pH 6.7, pH 4.5 and pH 5.5, respectively.
5. RNase Pp-1, is a guanyloribonuclease [EC 2. 7. 7. 26].
6. RNase Pp-2 is a nonspecific RNase [EC 2. 7. 7. 17].
7. RNase Pp-3 may be classified as a nonspecific RNase [EC 2. 7. 7. 17], but it attacks purine nucleotide bonds preferentially.
