Homogeneity and Sequential Periodate Oxidation of a Glycopeptide from Taka-Amylase A

Abstract

The homogeneity of a glycopeptide obtained from Taka-amylase A [EC 5. 2. 1. 1], was reinvestigated by column chromatography on Dowcx 50×2 and high voltage paper electrophoresis. About 90 per cent of the glycopeptide was found to be homogeneous and to consist of one mole each of aspartic acid, ser ne and ammonia, 2 moles of N-acetylglucosamine and 6 moles of mannose.
Sequential periodate oxidation of the purified homogeneous glycopcptide indicated that its carbohydrate sequence is Man-Man-G1eNAc-G1cNAc-NH.
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