Abstract
Lipopolysaccharides (LPS) and haptenic polysaccharides produced by intergroup recombinants of Salmonella were investigated.
1. The O-antigenic activities and contents of specific sugars were much less in LPS preparations from SR type recombinants than in those from the parent (S form). LPS of R-type recombinants was entirely devoid of the parental activities and sugars of the O antigens.
2. Recombinants between S. abony (donor) and S. milwaukee (recipient) produced defective LPS, lacking glucosamine at the terminal position of the R-core.
3. An antigenicity common to R recombinants was demonstrated in the SR-LPS of the progenies of a cross between S. riogrande (donor) and S. poona (recipient), suggesting that the R-core is exposed and can react with R-antibody owing to reduction in the number of O-side chains in the macromolecule.
4. Haptenic polysaccharides with O-antigenic specificities of the recipients were found in extracts of the SR recombinants. These substances were isolated and shown immunologically and biochemically to have polymerized O-side chains.
