In vitro Conversion of 7α-Hydroxycholesterol to Some Natural C₂₄-Bile Acids with Special Reference to Chenodeoxycholic Acid Biogenesis

Abstract

7α-Hydroxycholesterol-¹⁴C was incubated with fortified rat mitochondria and the following C₂₄-bile acids were identified: 3β, 7α-dihydroxychol-5-enoic acid, chenodeoxycholic acid and α-muricholic acid.
From these and previous data the following new pathway of chenodeoxycholic acid biogenesis is proposed: cholesterol→7α-hydroxycholesterol→3β, 7α-dihydroxychol-5-enoic acid→ chenodeoxycholic acid.