Studies on the Substrate Specificity of Taka-amylase A

VIII. Synthesis of Phenyl 6-Fluoro-6-deoxy-α-maltoside and It's Enzymatic Susceptibility

Abstract

The synthesis of phenyl 6-fluoro-6-deoxy-α-maltoside is described. The compound was found to be much less susceptible to Taka-amylase A [EC 3. 2. 1. 1] than phenyl α-maltoside. This indicates that the hydroxyl group at position-6 of phenyl α-maltoside is important for the enzymatic reaction.
1, 2, 3, 4-Tetra-O-acetyl-6-fluoro-6-deoxy-glucose, a reference compound in gas chromatographic analyses, was synthesized by a new method.