Partial Digestion with Ribonuclese T₁ and Derivation of the Complete Sequence of Tyrosine Transfer Ribonucleic Acid from Torulopsis utilis

Abstract

Several large oligonucleotide fragments obtained by partial digestion of tyrosine transfer RNA from Torulopsis utilis with ribonuclease T₁ [EC 2. 7. 7. 26] were separated and their sequences were determined. The results of these analyses and those described in a preceding paper permitted the derivation of the complete primary structure of this tRNA. The established sequence was compared with those of other transfer RNAs of T. utilis and of different species for an understanding of the structural basis of functions of transfer RNA.