The Metabolic Sequence for the Occurrence of an Anomalous Bile Acid, 12-Ketochenodeoxycholic Acid, Found in the Bile of Hepatobiliary Diseased Patients
1972 Volume 72 Issue 1 Pages 165-172
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1972 Volume 72 Issue 1 Pages 165-172
The metabolic sequence for the occurrence of an anomalous bile acid, 12-ketoehenodeoxycholic acid (12-KCCA), * found in the bile of patients with hepatobiliary diseases was disclosed.
1. The H-l strain of Staphylococcus epidermidis isolated from the fistula bile of a patient, in which conjugated 12-KCCA was detected, showed fairly strict substrate specificity for 12α-hydroxysteroid dehydrogenation among bile acids and alcohols.
2. Only the H-l strain had the ability to dehydrogenate cholic acid at 12-position, but a hundred strains of Staph. epidermidis collected either from nasal cavities or indoor air did not.
3. Labeled 12-KCCA administered per os to rats remained unchanged in the intestinal cavity, while it was converted predominantly to taurocholate during the enterohepatic circulation.
4. The 12-ketosteroid reductase was shown to be present in the rat liver homogenate and to localize predominantly in the microsomal fraction.
5. Based on these observations, a possible metabolic sequence was presented.
