1972 Volume 72 Issue 1 Pages 39-48
Biosynthesis of proteins of R-type pyocins, phage tail-like bacteriocins produced by Pseudomonas aeruginosa, and their assembly into pyocin particles were studied. Production of pyocins was induced with mitomycin C. Pyocin proteins appeared at 10-15min after induction and active pyocin particles appeared about lOmin later. A similar time lag, probably necessary for assembly of subunit proteins, was also observed during the exponential production of pyocin and confirmed by a pulselabelling experiment. By centrifugation on sucrose density gradient a particle with sedimentation coefficient of 41S was observed during the course of pyocin production. Some of its properties were investigated and discussed.
