3-Acetoxy-1-acetyl-5-methylpyrazole, a Novel Acetylating Reagent of Protein
1972 Volume 72 Issue 1 Pages 65-72
Details
1972 Volume 72 Issue 1 Pages 65-72
1. The reactivity of 3-acetoxy-1-acetyl-5-methylpyrazole (AAMP) with amino and hydroxyl groups of proteins has been studied.
2. AAMP was rather stable in an aqueous solution (pH 7.5) than N-acetylimidazole.
3. AAMP was very reactive towards amino groups of protein at pH 7.5 and at 27 or 37°C. AAMP was also reactive towards aliphatic and phenolic hydroxyl groups of proteins, but the reaction of AAMP towards amino groups was also observed to some extent.
4. AAMP was not so specific as N-acetylimidazole towards free tyrosyl residues of proteins.
5. From the experiments described here, it was concluded that AAMP could be used as a novel acetylating reagent for amino groups of proteins.
