Proteolipid of Bovine Brain White Matter:Phospholipid Components

Abstract

1. Proteolipid was prepared from bovine brain white matter by a method involving fluff formation. The preparation was soluble in chloroform: methanol (2: 1 and 1: 1, v/v) but insoluble in acetone, ether, and ethanol.
2. The lipid composition of the proteolipid was determined by stepwise hydrolysis and characterized by a large amount of sphingomyelin.
3. Much of the lipid portion, containing most of the sphingomyelin, was removed by treating the proteolipid with a mixture of ethanol and ether. The residual portion was still soluble in chloroform: methanol (2: 1, v/v) and was found to have a specific affinity to sphingomyelin.