Abstract
1. In the presence of 2mg% of phosphotungstic acid (PTA), the release by AMP of the controlled respiration of rat liver mitochondria was inhibited, while that by ADP was not. Decreasing the concentration of PTA to 1mg%, the release by AMP occurred after a definite lag period.
2. In the presence of 2 mg% of PTA, the formation of ADP from AMP and ATP by intact rat liver mitochondria was inhibited, while AMP and ATP were formed from ADP regardless of the presence of PTA.
3. Incorporation studies using radioactive AMP and ADP indicated that penetration into the mitochondria was markedly inhibited by PTA for AMP but only slightly for ADP, explaining the above results.
4. These results suggest that PTA affects a site between the outer membrane and the adenylate kinase [EC 2. 7. 4. 3] loci in rat liver mitochondria.
