Abstract
Using synthetic sulfated polysaccharides (SP's) of relatively low molecular weight, the effects of SP's on lipoprotein lipase [EC 3. 1. 1. 3] (LPL) were examined.
(1) The activities of synthetic SP's in the liberation of LPL are proportional to their activities for the inhibition of the LPL activity.
(2) The activities of these SP's in the liberation of LPL both in vivo and in vitro, and for the inhibition of the LPL activity are proportional to their sulfate contents and molecular weights.
(3) The chemical nature of the repeating a sugar units and the type of glycosidic linkage in synthetic SP's do not appreciably influence their activities in the liberation of LPL or for the inhibition of the LPL activity.
(4) Unlike synthetic SP's, heparin has a specific effect on the liberation of LPL only in vivo.
(5) The release of LPL from adipose tissue in vitro increases with increase in the ionic strength of the incubation medium.
(6) The mechanism whereby SP's liberate LPL into the blood stream is discussed on the basis of these results.
