Studies on Ribosomal Ribonucleic Acid from Yeast

II. Specific Complex of Yeast Ribosomal RNA

Abstract

1. The anomalous heavier 30S RNA extracted from the ribosomes of baker's yeast (Saccharomyces cerevisiae) is considered to be a hydrogen-bonded complex of one molecule each of 26 and 18S rRNA.
2. Addition of EDTA during the preparation of RNA from ribosomes by the bentonite-phenol method results in the extraction of a relatively large amount of 30S RNA. Treatment of a mixed solution of isolated 26 and 18S rRNA with phenol, in the presence of EDTA, failed to provide evidence for the formation of 30S RNA.
3. A specific complex is formed between 18 and 26S rRNA in a buffer containing 10mM Mg²⁺.