2, 3-Diphosphoglycerate and Phosphoglycerate Mutase Levels in Animal Tissues and Erythrocytes

Abstract

A direct method for simultaneously estimating the activity of phosphoglycerate mutase [EC 2. 7. 5. 3] and the concentration of 2, 3-diphosphoglycerate in tissue homogenates and in the hemolysates of mammals was established. By this method, a marked increase in the activity of phosphoglycerate mutase was observed in the red cells of patients with anemia and congestive heart failure, while the concentration of 2, 3-diphosphoglycerate was in the normal range.