Isolation and Characterization of Glycopeptides from Rat Liver Mitochondria

Abstract

Glycopeptides were prepared by pronase digestion from rat liver mitochondria, and were fractionated into neutral and acidic fractions. Neutral glycopeptides accounted for 20-30% of the total glycopeptides on a molar basis, and their carbohydrate moieties consisted mainly of glucosamine and mannose. Acidic glycopeptides consisted mainly of glucosamine, sialic acid, mannose, galactose, and fucose. These mitochondrial glycopeptides were different from the corresponding glycopeptides from hepatic microsomes in size and chemical composition.