Diphenyl Phosphorazidate: A New Potent Organic Azide Inhibiting Energy Transformation Reactions

Abstract

Diphenyl phosphorazidate, a new organic azide, was found to be a potent inhibitor for certain energy transformation reactions. It inhibits NaN₃-sensitive, membranebound ATPase [EC 3. 6. 1. 3] of Escherichia coli and the synthesis of ATP without interfering with succinate oxidation by intact cells. It inhibits selectively the active transport reaction for isoleucine, not for proline, by intact E. coli cells.