Tryptic Peptides from the β Polypeptide Chain of AII Component of Chicken Hemoglobin

Abstract

The aminoethylated β polypeptide chain in AII component from the hemoglobin of adult chicken was digested with trypsin [EC 3. 4. 21. 4] and the resulting peptides were separated and purified by ion exchange chromatography, paper chromatography, and gel filtration. Eighteen tryptic peptides, which were nonoverlapping, accounted for all of the amino acid residues in the β polypeptide chain. The amino acid sequences of the tryptic peptides were established by a combination of enzymatic digestion and subtractive Edman degradation.