Enzyme Immunoassay of Testosterone Using the Testosterone-Glucoamylase Comples

Abstract

A highly sensitive, reproducible method was established for enzyme-coupled im-munoassay of testosterone, involving coupling of testosterone to glucoamylase [EC 3.2.1.3] with a water-soluble coupling reagent carbodiimide. No enzyme activity was lost during this coupling procedure. The sensitivity of the method was comparable to that of competitive protein binding assay.