Sulfate Incorporation from Ascorbate 2-Sulfate into Chondroitin Sulfate by Embryonic Chick Cartilage Epiphyses
1976 Volume 80 Issue 6 Pages 1215-1221
Details
1976 Volume 80 Issue 6 Pages 1215-1221
Radioactivity was significantly incorporated from ascorbate 2-[35S] sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses. The extent of incorporation was comparable with that from inorganic [35S]sulfate. The radioactive chondroitin sulfate formed from ascor-bate 2-[35S]sulfate gave two radioactive disaccharides on chondroitinase-ABC [EC 4.2.2.4] digestion.
The incorporation was markedly decreased by inorganic sulfate. The time course of incorporation from ascorbate 2-[35S]sulfate and inorganic [35S]sulfate into chondroitin sulfate and the constituent disaccharides suggest that the incorporation rates from the two radioactive substances are different.
