Incorporation of [¹⁴C] Glucose from UDP [¹⁴C] Glucose into a Phosphoglycolipid by Cell-free Particulate Systems of a Moderately Halophilic Gram-negative Bacterium, Pseudomonas halosaccharolytica ATCC 29423

Abstract

A glucosyl group from uridine diphosphate [U-¹⁴C]glucose is incorporated into a phosphoglycolipid, probably a glucosylphosphatidylglycerol, by a disrupted membrane enzyme prepa-ration from a gram-negative, moderately halophilic bacterium, Pseudomonas halosaccharolytica ATCC 29423. The conversion of [¹⁴C]phosphatidylglycerol into phosphoglycolipid by the particulate preparation was also enhanced in the presence of non-labelled UDP-glucose. A chemical degradation study of labelled phosphoglycolipid showed the bulk of the radioactivity from UDP [U-¹⁴C]glucose to be associated with the glucose moiety, which also appeared to be attached to the hydroxyl group of a second glycerol.