The Site-specific Deoxyribonuclease from Bacillus pumilus (Endonuclease R. Bpu1387)

Abstract

A new site-specific endonuclease (DNase) was isolated from the cells of Bacillus pumilus AHU 1387 strain. This enzyme (endonuclease R.Bpu 1387) introduced double-stranded scissions at unique sites on DNA's of coli phage λ, λdvl, coil phage T7, Bacillus phage φ105C, Bacillus phage SP10, and Simian Virus 40, in the presence of magnesium ion. The activity was stimulated by the presence of NaCl.