Abstract
Two Lifschütz-positive C24-bile acids were isolated from bladder bile of hens. One of these was identified by isotope dilution experiments after conversion to a 3H-labeled compound, and also by GLC after methoxylation, as 3β, 7α-dihydroxychol-5-enoic acid, a key intermediate of chenodeoxycholic acid biogenesis. The other, to which the structure 3β, 7α-dihydroxychol-4-enoic acid had been assigned previously, was proved to be its 3α-epimer by several experiments.
These findings favor the alternative pathway of chenodeoxycholic acid biogenesis proposed by Yamasaki and his associates.
