Nucleotide Sequence of Initiator tRNA from Bacillus subtilis

Abstract

Initiator methionine tRNA, tRNA^Met_f was purified from Bacillus subtilis W168 by the use of two column chromatographies on DEAE-Sephadex A-50 and BD-cellulose. The nucleotidesequence was determined to be pC-G-C-G-G-G-G-U-G-G-A-G-C-A-G-U-U-C-G-G-D-A-G-C-U-C-G-U-C-G-G-G-C-U-C-A-U-A-A-C-C-C-G-A-A-G-G-U-C-G-C-A-G-G-T-Ψ-C-A-A-A-U-C-C-U-G-C-C-C-C-C-G-C-A-A-C-C-AOH. This tRNA^Met_f exhibited a melting temperature 8°C lower than that of E. coli tRNA^Met_f in the presence of 0.01 M magnesium acetate.