Alterations in Glucocorticoid Receptor Conformation by Molybdate

Abstract

Glucocorticoid receptor activities in cytosol from lactating rat mammary glands were greatly stabilized by molybdate against inactivation during incubation at 25°C. The sedimentation coefficient was 9-10 S in the presence of 10mM Na₂MoO₄, but 6-7 S in the absence of the ion. These data strongly suggest that alterations in receptor conformation induced by direct interaction with molybdate are involved in the stabilization process.