Structural Study of the Carbohydrate Moiety of Bovine Pancreatic Ribonuclease B

Abstract

The sugar chain of bovine pancreatic ribonuclease B was released from the polypeptide moiety by hydrazinolysis and by endo-β-N-acetylglucosaminidase H digestion, and reduced with NaB[³H]₄ after N-acetylation. The radioactive oligosaccharide mixtures thus obtained were fractionated by Bio-Gel P-4 column chromatography, and their structures were studied by sequential exoglycosidase digestion, methylation study, periodate oxidation and acetolysis. The results show that bovine pancreatic ribonuclease B has one of the following sugar chains as asparagine-linked carbohydrate.