Analysis of Cholesterol in High Density Lipoprotein Subfractions by High Performance Liquid Chromatography

Abstract

We succeeded in the separation of human serum high density lipoprotein into their subfractions (HDL₂ and HDL₃) by high performance liquid chromatography with gel permeation columns (1, 2). The cholesterol contents in HDL₂ and HDL₃ for normal men and women (n=42) and for the patients with atherosclerotic heart disease (n=20) were measured by this separation method. The results in our experiment agree closely with those reported by other investigators using the ultracentrifugal method.