A Simple Peptide Fractionation by Hydrophobic Chromatography with a Prepacked Reversed-Phase Column
1981 Volume 89 Issue 2 Pages 437-441
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1981 Volume 89 Issue 2 Pages 437-441
A simple and highly reproducible liquid chromatographic procedure was developed to fractionate peptides by using a prepacked reversed-phase column (RP-8) at low pressure. Peptides were eluted in 0.1% conc. HCl by using a gradient of ethanol in order of increasing hydrophobicity.
