Identification of the Trimannosyl-Chitobiose Structure in Sugar Moieties of Japanese Quail Ovomucoid

Abstract

Ovomucoid isolated from egg white of japanese quail contained mannose, galactose, glucosamine, and sialic acid (8.6:1.3:6.6:0.5 mol/mol) as sugar components. Sugar chains were liberated from the polypeptide portion by hydrazinolysis, N-acetylated and fractionated on Bio-Gel P-2. The reducing ends of the thus obtained sugar moieties were pyridylaminated. Chemical and exoglycosidase digestion studies of the fluorescent pyridylamino derivative of a major sugar fraction indicated the following structure for one of the major sugar chains of japanese quail ovomucoid; Manα1→6 (Manα1→3) Manβ1→4 GlcNAcβ1→4 GlcNAc.