Study on the Interaction between Hemoglobin Izu (Maraca) and Organic Phosphates by Spin Labeling

Abstract

ESR spectra of the carbonmonoxy, oxy, and deoxy derivatives of hemoglobin Izu [Hb Izu (Macaca): β 83 (EF 7) Gly→Cys] labeled at cysteine β 83 with maleimide spin label have been observed in the presence and absence of 2, 3-diphosphoglycerate and inositol hexaphosphate. The τ_c, values obtained from the spectra indicated that inositol hexaphosphate binds to all the derivatives of Hb Izu, but 2, 3-diphosphoglycerate only to the deoxy derivatives.