The Inhibitory Mode of Aphidicolin on DNA Synthesis in NaCl-Treated HeLa Cell Nuclei

Abstract

Isolated HeLa cell nuclei were treated with NaCl at various concentrations and inhibition by aphidicolin of DNA synthesis in the treated nuclei was studied. The inhibition was either noncompetitive or of the mixed type with respect to each dNTP when the nuclei were treated with NaCl at concentrations lower than 0.08M. However, aphidicolin was a competitive inhibitor with respect to dCTP and a noncompetitive or mixed type inhibitor with respect to the other 3 dNTPs when they were treated with NaCl at concentrations higher than 0.1M. These results suggest the presence of nuclear factor (s) responsible for the changes in the inhibitory mode of aphidicolin on endogenous nuclear DNA synthesis.